EMB Agar

Mã: M317

Xuất xứ: Himedia-Ấn Độ

Quy cách: chai 500g

Đơn vị tính: chai

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Thông tin:

Composition** Ingredients Gms / Litre Peptic digest of animal tissue 10.000 Dipotassium phosphate 2.000 Lactose 5.000 Sucrose 5.000 Eosin - Y 0.400 Methylene blue 0.065 Agar 13.500 Final pH ( at 25°C) 7.4±0.2 Directions Suspend 35.96 grams in 1000 ml distilled water. Mix until suspension is uniform. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. AVOID OVERHEATING. Cool to 45-50°C and shake the medium in order to oxidize the methylene blue (i.e. to restore its blue colour) and to suspend the flocculent precipitate. (If EMB Agar is inoculated on the same day, it may be used without autoclave sterilization). Precaution : Store the medium away from light to avoid photooxidation pH 7.00-7.40 Storage and Shelf Life : Store below 30°C in a tightly closed container and the prepared medium at 2 - 8°C

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