Baird Parker Agar

Composition** Ingredients Gms / Litre Tryptone 10.000 HM Peptone B# 5.000 Yeast extract 1.000 Glycine 12.000 Sodium puruvate 10.000 Lithium chloride 5.000 Agar 20.000 Final pH ( at 25°C) 7.0±0.2 Directions Suspend 63.0 grams in 950 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 50°C and aseptically add 50 ml concentrated Egg Yolk Emulsion (FD045) and 3 ml sterile 3.5% Potassium Tellurite solution (FD047) or 50 ml Egg Yolk Tellurite Emulsion (FD046). For additional selectivity, if desired add rehydrated contents of 1 vial of BP Sulpha Supplement (FD069). Alternatively 1 vial of Fibrinogen Plasma Trypsin Inhibitor Supplement (FD195) may be used per 90 ml medium in place of Egg yolk Tellurite Emulsion (FD046) for identification of coagulase, positive Stapylococci. Mix well and pour into sterile Petri plates.. pH 6.80-7.20 Storage and Shelf Life : Store below 30°C in a tightly closed container and the prepared medium at 2 - 8°C